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Theprotocolsforpreparingcompetentcellsvarybychoiceoftransformationmethod—heatshockorelectroporation.Heatshocktransformationisalsoknownas ...,Placetransformationtubeintoa42°Cwaterbathfor42seconds(30-60sec).,,Heat-shockthecellsfor45secondsat42°Cwit...
Heat shock transformation (E.coli) protocol
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